图观察规律或结论:从根际-根表-根内,细菌的多样性逐渐下降的。不同地区的差别小于不同部分的差别。
附图注原文:
(B) Within sample diversity (α-diversity) measurements between rhizospheric compartments indicate a decreasing gradient in microbial diversity from the rhizosphere to the endosphere independent of soil type. Estimated species richness was calculated as eShannon_entropy. The horizontal bars within boxes represent median. The tops and bottoms of boxes represent 75th and 25th quartiles, respectively. The upper and lower whiskers extend 1.5× the interquartile range from the upper edge and lower edge of the box, respectively. All outliers are plotted as individual points.
示例2
Beckers, B., et al. (2017). Microbiome 5(1): 25.
这篇文章分析了白杨树不同区域的细菌组成和差异,16S分析中非常中规中矩,且而没有任何后续实验,但在今年还能发这么好的杂志,大家可以分析一下原因。
附图注原文:
Fig. 2 Alpha diversity estimates of the bacterial communities. a OTU richness estimates (number of observed OTUs). b Pielou’s evenness estimates. c Inverse Simpson diversity indices. Box plots display the first (25%) and third (75%) quartiles, the median and the maximum and minimum observed values within each data set. Alpha diversity estimates represent 15 biological replicates for the rhizosphere soil and root samples and 11 replicates for the stem and leaf samples and were calculated in mothur with 10,000 iterations. Data were analyzed by means of one-way ANOVAs and Tukey-Kramer post hoc comparisons. The overall plant compartment effects (F(DFn, DFd) and P value) are displayed at the top of each graph. Significant differences (P < 0.05) across plant compartments are indicated with lowercase letters
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Beckers, B., et al. (2017). “Structural variability and niche differentiation in the rhizosphere and endosphere bacterial microbiome of field-grown poplar trees.” Microbiome 5(1): 25.
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