基于扩增子测序的新冠病毒测序引物设计

nCoV-2019_1_LEFT

nCoV-2019_1

ACCAACCAACTTTCGATCTCTTGT

24

41.67

60.69

nCoV-2019_1_RIGHT

nCoV-2019_1

CATCTTTAAGATGTTGACGTGCCTC

25

44

60.45

nCoV-2019_2_LEFT

nCoV-2019_2

CTGTTTTACAGGTTCGCGACGT

22

50

61.67

nCoV-2019_2_RIGHT

nCoV-2019_2

TAAGGATCAGTGCCAAGCTCGT

22

50

61.74

nCoV-2019_3_LEFT

nCoV-2019_1

CGGTAATAAAGGAGCTGGTGGC

22

54.55

61.32

nCoV-2019_3_RIGHT

nCoV-2019_1

AAGGTGTCTGCAATTCATAGCTCT

24

41.67

60.32

nCoV-2019_4_LEFT

nCoV-2019_2

GGTGTATACTGCTGCCGTGAAC

22

54.55

61.56

nCoV-2019_4_RIGHT

nCoV-2019_2

CACAAGTAGTGGCACCTTCTTTAGT

25

44

60.97

nCoV-2019_5_LEFT

nCoV-2019_1

TGGTGAAACTTCATGGCAGACG

22

50

61.39

nCoV-2019_5_RIGHT

nCoV-2019_1

ATTGATGTTGACTTTCTCTTTTTGGAGT

28

32.14

60.17

nCoV-2019_6_LEFT

nCoV-2019_2

GGTGTTGTTGGAGAAGGTTCCG

22

54.55

61.64

nCoV-2019_6_RIGHT

nCoV-2019_2

TAGCGGCCTTCTGTAAAACACG

22

50

61.18

nCoV-2019_7_LEFT

nCoV-2019_1

ATCAGAGGCTGCTCGTGTTGTA

22

50

61.73

nCoV-2019_7_LEFT_alt0

nCoV-2019_1

CATTTGCATCAGAGGCTGCTCG

22

54.55

62.44

nCoV-2019_7_RIGHT

nCoV-2019_1

TGCACAGGTGACAATTTGTCCA

22

45.45

60.95

nCoV-2019_7_RIGHT_alt5

nCoV-2019_1

AGGTGACAATTTGTCCACCGAC

22

50

61.07

nCoV-2019_8_LEFT

nCoV-2019_2

AGAGTTTCTTAGAGACGGTTGGGA

24

45.83

61

nCoV-2019_8_RIGHT

nCoV-2019_2

GCTTCAACAGCTTCACTAGTAGGT

24

45.83

60.56

nCoV-2019_9_LEFT

nCoV-2019_1

TCCCACAGAAGTGTTAACAGAGGA

24

45.83

61.18

nCoV-2019_9_LEFT_alt4

nCoV-2019_1

TTCCCACAGAAGTGTTAACAGAGG

24

45.83

60.44

nCoV-2019_9_RIGHT

nCoV-2019_1

ATGACAGCATCTGCCACAACAC

22

50

61.71

nCoV-2019_9_RIGHT_alt2

nCoV-2019_1

GACAGCATCTGCCACAACACAG

22

54.55

62.26

nCoV-2019_10_LEFT

nCoV-2019_2

TGAGAAGTGCTCTGCCTATACAGT

24

45.83

61.12

nCoV-2019_10_RIGHT

nCoV-2019_2

TCATCTAACCAATCTTCTTCTTGCTCT

27

37.04

60.31

nCoV-2019_11_LEFT

nCoV-2019_1

GGAATTTGGTGCCACTTCTGCT

22

50

61.66

nCoV-2019_11_RIGHT

nCoV-2019_1

TCATCAGATTCAACTTGCATGGCA

24

41.67

61.35

nCoV-2019_12_LEFT

nCoV-2019_2

AAACATGGAGGAGGTGTTGCAG

22

50

61.08

nCoV-2019_12_RIGHT

nCoV-2019_2

TTCACTCTTCATTTCCAAAAAGCTTGA

27

33.33

60.36

nCoV-2019_13_LEFT

nCoV-2019_1

TCGCACAAATGTCTACTTAGCTGT

24

41.67

60.56

nCoV-2019_13_RIGHT

nCoV-2019_1

ACCACAGCAGTTAAAACACCCT

22

45.45

60.36

nCoV-2019_14_LEFT

nCoV-2019_2

CATCCAGATTCTGCCACTCTTGT

23

47.83

60.62

nCoV-2019_14_LEFT_alt4

nCoV-2019_2

TGGCAATCTTCATCCAGATTCTGC

24

45.83

61.47

nCoV-2019_14_RIGHT

nCoV-2019_2

AGTTTCCACACAGACAGGCATT

22

45.45

60.42

nCoV-2019_14_RIGHT_alt2

nCoV-2019_2

TGCGTGTTTCTTCTGCATGTGC

22

50

62.76

nCoV-2019_15_LEFT

nCoV-2019_1

ACAGTGCTTAAAAAGTGTAAAAGTGCC

27

37.04

61.32

nCoV-2019_15_LEFT_alt1

nCoV-2019_1

AGTGCTTAAAAAGTGTAAAAGTGCCT

26

34.62

60.13

nCoV-2019_15_RIGHT

nCoV-2019_1

AACAGAAACTGTAGCTGGCACT

22

45.45

60.16

nCoV-2019_15_RIGHT_alt3

nCoV-2019_1

ACTGTAGCTGGCACTTTGAGAGA

23

47.83

61.57

nCoV-2019_16_LEFT

nCoV-2019_2

AATTTGGAAGAAGCTGCTCGGT

22

45.45

60.82

nCoV-2019_16_RIGHT

nCoV-2019_2

CACAACTTGCGTGTGGAGGTTA

22

50

61.32

nCoV-2019_17_LEFT

nCoV-2019_1

CTTCTTTCTTTGAGAGAAGTGAGGACT

27

40.74

60.69

nCoV-2019_17_RIGHT

nCoV-2019_1

TTTGTTGGAGTGTTAACAATGCAGT

25

36

60.11

nCoV-2019_18_LEFT

nCoV-2019_2

TGGAAATACCCACAAGTTAATGGTTTAAC

29

34.48

60.69

nCoV-2019_18_LEFT_alt2

nCoV-2019_2

ACTTCTATTAAATGGGCAGATAACAACTGT

30

33.33

61.38

nCoV-2019_18_RIGHT

nCoV-2019_2

AGCTTGTTTACCACACGTACAAGG

24

45.83

61.51

nCoV-2019_18_RIGHT_alt1

nCoV-2019_2

GCTTGTTTACCACACGTACAAGG

23

47.83

60.3

nCoV-2019_19_LEFT

nCoV-2019_1

GCTGTTATGTACATGGGCACACT

23

47.83

61.18

nCoV-2019_19_RIGHT

nCoV-2019_1

TGTCCAACTTAGGGTCAATTTCTGT

25

40

60.4

nCoV-2019_20_LEFT

nCoV-2019_2

ACAAAGAAAACAGTTACACAACAACCA

27

33.33

60.68

nCoV-2019_20_RIGHT

nCoV-2019_2

ACGTGGCTTTATTAGTTGCATTGTT

25

36

60.28

nCoV-2019_21_LEFT

nCoV-2019_1

TGGCTATTGATTATAAACACTACACACCC

29

37.93

61.49

nCoV-2019_21_LEFT_alt2

nCoV-2019_1

GGCTATTGATTATAAACACTACACACCCT

29

37.93

61.29

nCoV-2019_21_RIGHT

nCoV-2019_1

TAGATCTGTGTGGCCAACCTCT

22

50

60.83

nCoV-2019_21_RIGHT_alt0

nCoV-2019_1

GATCTGTGTGGCCAACCTCTTC

22

54.55

61.2

nCoV-2019_22_LEFT

nCoV-2019_2

ACTACCGAAGTTGTAGGAGACATTATACT

29

37.93

61.25

nCoV-2019_22_RIGHT

nCoV-2019_2

ACAGTATTCTTTGCTATAGTAGTCGGC

27

40.74

60.73

nCoV-2019_23_LEFT

nCoV-2019_1

ACAACTACTAACATAGTTACACGGTGT

27

37.04

60.26

nCoV-2019_23_RIGHT

nCoV-2019_1

ACCAGTACAGTAGGTTGCAATAGTG

25

44

60.57

nCoV-2019_24_LEFT

nCoV-2019_2

AGGCATGCCTTCTTACTGTACTG

23

47.83

60.37

nCoV-2019_24_RIGHT

nCoV-2019_2

ACATTCTAACCATAGCTGAAATCGGG

26

42.31

61.19

nCoV-2019_25_LEFT

nCoV-2019_1

GCAATTGTTTTTCAGCTATTTTGCAGT

27

33.33

60.73

nCoV-2019_25_RIGHT

nCoV-2019_1

ACTGTAGTGACAAGTCTCTCGCA

23

47.83

61.3

nCoV-2019_26_LEFT

nCoV-2019_2

TTGTGATACATTCTGTGCTGGTAGT

25

40

60.28

nCoV-2019_26_RIGHT

nCoV-2019_2

TCCGCACTATCACCAACATCAG

22

50

60.42

nCoV-2019_27_LEFT

nCoV-2019_1

ACTACAGTCAGCTTATGTGTCAACC

25

44

60.8

nCoV-2019_27_RIGHT

nCoV-2019_1

AATACAAGCACCAAGGTCACGG

22

50

61.13

nCoV-2019_28_LEFT

nCoV-2019_2

ACATAGAAGTTACTGGCGATAGTTGT

26

38.46

60.13

nCoV-2019_28_RIGHT

nCoV-2019_2

TGTTTAGACATGACATGAACAGGTGT

26

38.46

60.91

nCoV-2019_29_LEFT

nCoV-2019_1

ACTTGTGTTCCTTTTTGTTGCTGC

24

41.67

61.39

nCoV-2019_29_RIGHT

nCoV-2019_1

AGTGTACTCTATAAGTTTTGATGGTGTGT

29

34.48

60.69

nCoV-2019_30_LEFT

nCoV-2019_2

GCACAACTAATGGTGACTTTTTGCA

25

40

61.19

nCoV-2019_30_RIGHT

nCoV-2019_2

ACCACTAGTAGATACACAAACACCAG

26

42.31

60.3

nCoV-2019_31_LEFT

nCoV-2019_1

TTCTGAGTACTGTAGGCACGGC

22

54.55

62.03

nCoV-2019_31_RIGHT

nCoV-2019_1

ACAGAATAAACACCAGGTAAGAATGAGT

28

35.71

60.69

nCoV-2019_32_LEFT

nCoV-2019_2

TGGTGAATACAGTCATGTAGTTGCC

25

44

61.09

nCoV-2019_32_RIGHT

nCoV-2019_2

AGCACATCACTACGCAACTTTAGA

24

41.67

60.56

nCoV-2019_33_LEFT

nCoV-2019_1

ACTTTTGAAGAAGCTGCGCTGT

22

45.45

61.58

nCoV-2019_33_RIGHT

nCoV-2019_1

TGGACAGTAAACTACGTCATCAAGC

25

44

61.08

nCoV-2019_34_LEFT

nCoV-2019_2

TCCCATCTGGTAAAGTTGAGGGT

23

47.83

61.02

nCoV-2019_34_RIGHT

nCoV-2019_2

AGTGAAATTGGGCCTCATAGCA

22

45.45

60.03

nCoV-2019_35_LEFT

nCoV-2019_1

TGTTCGCATTCAACCAGGACAG

22

50

61.39

nCoV-2019_35_RIGHT

nCoV-2019_1

ACTTCATAGCCACAAGGTTAAAGTCA

26

38.46

60.69

nCoV-2019_36_LEFT

nCoV-2019_2

TTAGCTTGGTTGTACGCTGCTG

22

50

61.44

nCoV-2019_36_RIGHT

nCoV-2019_2

GAACAAAGACCATTGAGTACTCTGGA

26

42.31

60.74

nCoV-2019_37_LEFT

nCoV-2019_1

ACACACCACTGGTTGTTACTCAC

23

47.83

60.93

nCoV-2019_37_RIGHT

nCoV-2019_1

GTCCACACTCTCCTAGCACCAT

22

54.55

61.48

nCoV-2019_38_LEFT

nCoV-2019_2

ACTGTGTTATGTATGCATCAGCTGT

25

40

60.86

nCoV-2019_38_RIGHT

nCoV-2019_2

CACCAAGAGTCAGTCTAAAGTAGCG

25

48

61.13

nCoV-2019_39_LEFT

nCoV-2019_1

AGTATTGCCCTATTTTCTTCATAACTGGT

29

34.48

61

nCoV-2019_39_RIGHT

nCoV-2019_1

TGTAACTGGACACATTGAGCCC

22

50

60.55

nCoV-2019_40_LEFT

nCoV-2019_2

TGCACATCAGTAGTCTTACTCTCAGT

26

42.31

61.25

nCoV-2019_40_RIGHT

nCoV-2019_2

CATGGCTGCATCACGGTCAAAT

22

50

62.09

nCoV-2019_41_LEFT

nCoV-2019_1

GTTCCCTTCCATCATATGCAGCT

23

47.83

60.75

nCoV-2019_41_RIGHT

nCoV-2019_1

TGGTATGACAACCATTAGTTTGGCT

25

40

60.75

nCoV-2019_42_LEFT

nCoV-2019_2

TGCAAGAGATGGTTGTGTTCCC

22

50

61.08

nCoV-2019_42_RIGHT

nCoV-2019_2

CCTACCTCCCTTTGTTGTGTTGT

23

47.83

60.69

nCoV-2019_43_LEFT

nCoV-2019_1

TACGACAGATGTCTTGTGCTGC

22

50

60.93

nCoV-2019_43_RIGHT

nCoV-2019_1

AGCAGCATCTACAGCAAAAGCA

22

45.45

61.14

nCoV-2019_44_LEFT

nCoV-2019_2

TGCCACAGTACGTCTACAAGCT

22

50

61.66

nCoV-2019_44_LEFT_alt3

nCoV-2019_2

CCACAGTACGTCTACAAGCTGG

22

54.55

60.67

nCoV-2019_44_RIGHT

nCoV-2019_2

AACCTTTCCACATACCGCAGAC

22

50

60.87

nCoV-2019_44_RIGHT_alt0

nCoV-2019_2

CGCAGACGGTACAGACTGTGTT

22

54.55

62.77

nCoV-2019_45_LEFT

nCoV-2019_1

TACCTACAACTTGTGCTAATGACCC

25

44

60.57

nCoV-2019_45_LEFT_alt2

nCoV-2019_1

AGTATGTACAAATACCTACAACTTGTGCT

29

34.48

60.94

nCoV-2019_45_RIGHT

nCoV-2019_1

AAATTGTTTCTTCATGTTGGTAGTTAGAGA

30

30

60.01

nCoV-2019_45_RIGHT_alt7

nCoV-2019_1

TTCATGTTGGTAGTTAGAGAAAGTGTGTC

29

37.93

61.53

nCoV-2019_46_LEFT

nCoV-2019_2

TGTCGCTTCCAAGAAAAGGACG

22

50

61.38

nCoV-2019_46_LEFT_alt1

nCoV-2019_2

CGCTTCCAAGAAAAGGACGAAGA

23

47.83

61.35

nCoV-2019_46_RIGHT

nCoV-2019_2

CACGTTCACCTAAGTTGGCGTA

22

50

60.86

nCoV-2019_46_RIGHT_alt2

nCoV-2019_2

CACGTTCACCTAAGTTGGCGTAT

23

47.83

61.17

nCoV-2019_47_LEFT

nCoV-2019_1

AGGACTGGTATGATTTTGTAGAAAACCC

28

39.29

61.42

nCoV-2019_47_RIGHT

nCoV-2019_1

AATAACGGTCAAAGAGTTTTAACCTCTC

28

35.71

60.06

nCoV-2019_48_LEFT

nCoV-2019_2

TGTTGACACTGACTTAACAAAGCCT

25

40

61.09

nCoV-2019_48_RIGHT

nCoV-2019_2

TAGATTACCAGAAGCAGCGTGC

22

50

60.74

nCoV-2019_49_LEFT

nCoV-2019_1

AGGAATTACTTGTGTATGCTGCTGA

25

40

60.57

nCoV-2019_49_RIGHT

nCoV-2019_1

TGACGATGACTTGGTTAGCATTAATACA

28

35.71

61.05

nCoV-2019_50_LEFT

nCoV-2019_2

GTTGATAAGTACTTTGATTGTTACGATGGT

30

33.33

60.59

nCoV-2019_50_RIGHT

nCoV-2019_2

TAACATGTTGTGCCAACCACCA

22

45.45

60.95

nCoV-2019_51_LEFT

nCoV-2019_1

TCAATAGCCGCCACTAGAGGAG

22

54.55

61.34

nCoV-2019_51_RIGHT

nCoV-2019_1

AGTGCATTAACATTGGCCGTGA

22

45.45

61.14

nCoV-2019_52_LEFT

nCoV-2019_2

CATCAGGAGATGCCACAACTGC

22

54.55

61.83

nCoV-2019_52_RIGHT

nCoV-2019_2

GTTGAGAGCAAAATTCATGAGGTCC

25

44

60.62

nCoV-2019_53_LEFT

nCoV-2019_1

AGCAAAATGTTGGACTGAGACTGA

24

41.67

60.69

nCoV-2019_53_RIGHT

nCoV-2019_1

AGCCTCATAAAACTCAGGTTCCC

23

47.83

60.31

nCoV-2019_54_LEFT

nCoV-2019_2

TGAGTTAACAGGACACATGTTAGACA

26

38.46

60.18

nCoV-2019_54_RIGHT

nCoV-2019_2

AACCAAAAACTTGTCCATTAGCACA

25

36

60.11

nCoV-2019_55_LEFT

nCoV-2019_1

ACTCAACTTTACTTAGGAGGTATGAGCT

28

39.29

61.43

nCoV-2019_55_RIGHT

nCoV-2019_1

GGTGTACTCTCCTATTTGTACTTTACTGT

29

37.93

60.54

nCoV-2019_56_LEFT

nCoV-2019_2

ACCTAGACCACCACTTAACCGA

22

50

60.49

nCoV-2019_56_RIGHT

nCoV-2019_2

ACACTATGCGAGCAGAAGGGTA

22

50

61.21

nCoV-2019_57_LEFT

nCoV-2019_1

ATTCTACACTCCAGGGACCACC

22

54.55

61.16

nCoV-2019_57_RIGHT

nCoV-2019_1

GTAATTGAGCAGGGTCGCCAAT

22

50

61.26

nCoV-2019_58_LEFT

nCoV-2019_2

TGATTTGAGTGTTGTCAATGCCAGA

25

40

61.44

nCoV-2019_58_RIGHT

nCoV-2019_2

CTTTTCTCCAAGCAGGGTTACGT

23

47.83

61.06

nCoV-2019_59_LEFT

nCoV-2019_1

TCACGCATGATGTTTCATCTGCA

23

43.48

61.42

nCoV-2019_59_RIGHT

nCoV-2019_1

AAGAGTCCTGTTACATTTTCAGCTTG

26

38.46

60.02

nCoV-2019_60_LEFT

nCoV-2019_2

TGATAGAGACCTTTATGACAAGTTGCA

27

37.04

60.53

nCoV-2019_60_RIGHT

nCoV-2019_2

GGTACCAACAGCTTCTCTAGTAGC

24

50

60.44

nCoV-2019_61_LEFT

nCoV-2019_1

TGTTTATCACCCGCGAAGAAGC

22

50

61.5

nCoV-2019_61_RIGHT

nCoV-2019_1

ATCACATAGACAACAGGTGCGC

22

50

61.25

nCoV-2019_62_LEFT

nCoV-2019_2

GGCACATGGCTTTGAGTTGACA

22

50

61.91

nCoV-2019_62_RIGHT

nCoV-2019_2

GTTGAACCTTTCTACAAGCCGC

22

50

60.35

nCoV-2019_63_LEFT

nCoV-2019_1

TGTTAAGCGTGTTGACTGGACT

22

45.45

60.16

nCoV-2019_63_RIGHT

nCoV-2019_1

ACAAACTGCCACCATCACAACC

22

50

61.85

nCoV-2019_64_LEFT

nCoV-2019_2

TCGATAGATATCCTGCTAATTCCATTGT

28

35.71

60.11

nCoV-2019_64_RIGHT

nCoV-2019_2

AGTCTTGTAAAAGTGTTCCAGAGGT

25

40

60.1

nCoV-2019_65_LEFT

nCoV-2019_1

GCTGGCTTTAGCTTGTGGGTTT

22

50

61.92

nCoV-2019_65_RIGHT

nCoV-2019_1

TGTCAGTCATAGAACAAACACCAATAGT

28

35.71

60.9

nCoV-2019_66_LEFT

nCoV-2019_2

GGGTGTGGACATTGCTGCTAAT

22

50

61.21

nCoV-2019_66_RIGHT

nCoV-2019_2

TCAATTTCCATTTGACTCCTGGGT

24

41.67

60.45

nCoV-2019_67_LEFT

nCoV-2019_1

GTTGTCCAACAATTACCTGAAACTTACT

28

35.71

60.43

nCoV-2019_67_RIGHT

nCoV-2019_1

CAACCTTAGAAACTACAGATAAATCTTGGG

30

36.67

60.4

nCoV-2019_68_LEFT

nCoV-2019_2

ACAGGTTCATCTAAGTGTGTGTGT

24

41.67

60.14

nCoV-2019_68_RIGHT

nCoV-2019_2

CTCCTTTATCAGAACCAGCACCA

23

47.83

60.31

nCoV-2019_69_LEFT

nCoV-2019_1

TGTCGCAAAATATACTCAACTGTGTCA

27

37.04

61.43

nCoV-2019_69_RIGHT

nCoV-2019_1

TCTTTATAGCCACGGAACCTCCA

23

47.83

61.14

nCoV-2019_70_LEFT

nCoV-2019_2

ACAAAAGAAAATGACTCTAAAGAGGGTTT

29

31.03

60.13

nCoV-2019_70_RIGHT

nCoV-2019_2

TGACCTTCTTTTAAAGACATAACAGCAG

28

35.71

60.27

nCoV-2019_71_LEFT

nCoV-2019_1

ACAAATCCAATTCAGTTGTCTTCCTATTC

29

34.48

60.54

nCoV-2019_71_RIGHT

nCoV-2019_1

TGGAAAAGAAAGGTAAGAACAAGTCCT

27

37.04

60.8

nCoV-2019_72_LEFT

nCoV-2019_2

ACACGTGGTGTTTATTACCCTGAC

24

45.83

61.04

nCoV-2019_72_RIGHT

nCoV-2019_2

ACTCTGAACTCACTTTCCATCCAAC

25

44

60.97

nCoV-2019_73_LEFT

nCoV-2019_1

CAATTTTGTAATGATCCATTTTTGGGTGT

29

31.03

60.29

nCoV-2019_73_RIGHT

nCoV-2019_1

CACCAGCTGTCCAACCTGAAGA

22

54.55

62.45

nCoV-2019_74_LEFT

nCoV-2019_2

ACATCACTAGGTTTCAAACTTTACTTGC

28

35.71

60.68

nCoV-2019_74_RIGHT

nCoV-2019_2

GCAACACAGTTGCTGATTCTCTTC

24

45.83

60.85

nCoV-2019_75_LEFT

nCoV-2019_1

AGAGTCCAACCAACAGAATCTATTGT

26

38.46

60.24

nCoV-2019_75_RIGHT

nCoV-2019_1

ACCACCAACCTTAGAATCAAGATTGT

26

38.46

60.69

nCoV-2019_76_LEFT

nCoV-2019_2

AGGGCAAACTGGAAAGATTGCT

22

45.45

60.76

nCoV-2019_76_LEFT_alt3

nCoV-2019_2

GGGCAAACTGGAAAGATTGCTGA

23

47.83

61.87

nCoV-2019_76_RIGHT

nCoV-2019_2

ACACCTGTGCCTGTTAAACCAT

22

45.45

60.42

nCoV-2019_76_RIGHT_alt0

nCoV-2019_2

ACCTGTGCCTGTTAAACCATTGA

23

43.48

60.69

nCoV-2019_77_LEFT

nCoV-2019_1

CCAGCAACTGTTTGTGGACCTA

22

50

60.75

nCoV-2019_77_RIGHT

nCoV-2019_1

CAGCCCCTATTAAACAGCCTGC

22

54.55

61.59

nCoV-2019_78_LEFT

nCoV-2019_2

CAACTTACTCCTACTTGGCGTGT

23

47.83

60.55

nCoV-2019_78_RIGHT

nCoV-2019_2

TGTGTACAAAAACTGCCATATTGCA

25

36

60.22

nCoV-2019_79_LEFT

nCoV-2019_1

GTGGTGATTCAACTGAATGCAGC

23

47.83

60.92

nCoV-2019_79_RIGHT

nCoV-2019_1

CATTTCATCTGTGAGCAAAGGTGG

24

45.83

60.62

nCoV-2019_80_LEFT

nCoV-2019_2

TTGCCTTGGTGATATTGCTGCT

22

45.45

60.89

nCoV-2019_80_RIGHT

nCoV-2019_2

TGGAGCTAAGTTGTTTAACAAGCG

24

41.67

60.02

nCoV-2019_81_LEFT

nCoV-2019_1

GCACTTGGAAAACTTCAAGATGTGG

25

44

61.24

nCoV-2019_81_RIGHT

nCoV-2019_1

GTGAAGTTCTTTTCTTGTGCAGGG

24

45.83

60.73

nCoV-2019_82_LEFT

nCoV-2019_2

GGGCTATCATCTTATGTCCTTCCCT

25

48

61.52

nCoV-2019_82_RIGHT

nCoV-2019_2

TGCCAGAGATGTCACCTAAATCAA

24

41.67

60.02

nCoV-2019_83_LEFT

nCoV-2019_1

TCCTTTGCAACCTGAATTAGACTCA

25

40

60.46

nCoV-2019_83_RIGHT

nCoV-2019_1

TTTGACTCCTTTGAGCACTGGC

22

50

61.33

nCoV-2019_84_LEFT

nCoV-2019_2

TGCTGTAGTTGTCTCAAGGGCT

22

50

61.61

nCoV-2019_84_RIGHT

nCoV-2019_2

AGGTGTGAGTAAACTGTTACAAACAAC

27

37.04

60.36

nCoV-2019_85_LEFT

nCoV-2019_1

ACTAGCACTCTCCAAGGGTGTT

22

50

61.03

nCoV-2019_85_RIGHT

nCoV-2019_1

ACACAGTCTTTTACTCCAGATTCCC

25

44

60.51

nCoV-2019_86_LEFT

nCoV-2019_2

TCAGGTGATGGCACAACAAGTC

22

50

61.07

nCoV-2019_86_RIGHT

nCoV-2019_2

ACGAAAGCAAGAAAAAGAAGTACGC

25

40

61.01

nCoV-2019_87_LEFT

nCoV-2019_1

CGACTACTAGCGTGCCTTTGTA

22

50

60.16

nCoV-2019_87_RIGHT

nCoV-2019_1

ACTAGGTTCCATTGTTCAAGGAGC

24

45.83

60.81

nCoV-2019_88_LEFT

nCoV-2019_2

CCATGGCAGATTCCAACGGTAC

22

54.55

61.58

nCoV-2019_88_RIGHT

nCoV-2019_2

TGGTCAGAATAGTGCCATGGAGT

23

47.83

61.4

nCoV-2019_89_LEFT

nCoV-2019_1

GTACGCGTTCCATGTGGTCATT

22

50

61.5

nCoV-2019_89_LEFT_alt2

nCoV-2019_1

CGCGTTCCATGTGGTCATTCAA

22

50

62.01

nCoV-2019_89_RIGHT

nCoV-2019_1

ACCTGAAAGTCAACGAGATGAAACA

25

40

60.91

nCoV-2019_89_RIGHT_alt4

nCoV-2019_1

ACGAGATGAAACATCTGTTGTCACT

25

40

60.74

nCoV-2019_90_LEFT

nCoV-2019_2

ACACAGACCATTCCAGTAGCAGT

23

47.83

61.58

nCoV-2019_90_RIGHT

nCoV-2019_2

TGAAATGGTGAATTGCCCTCGT

22

45.45

60.82

nCoV-2019_91_LEFT

nCoV-2019_1

TCACTACCAAGAGTGTGTTAGAGGT

25

44

60.93

nCoV-2019_91_RIGHT

nCoV-2019_1

TTCAAGTGAGAACCAAAAGATAATAAGCA

29

31.03

60.03

nCoV-2019_92_LEFT

nCoV-2019_2

TTTGTGCTTTTTAGCCTTTCTGCT

24

37.5

60.14

nCoV-2019_92_RIGHT

nCoV-2019_2

AGGTTCCTGGCAATTAATTGTAAAAGG

27

37.04

60.53

nCoV-2019_93_LEFT

nCoV-2019_1

TGAGGCTGGTTCTAAATCACCCA

23

47.83

61.59

nCoV-2019_93_RIGHT

nCoV-2019_1

AGGTCTTCCTTGCCATGTTGAG

22

50

60.55

nCoV-2019_94_LEFT

nCoV-2019_2

GGCCCCAAGGTTTACCCAATAA

22

50

60.56

nCoV-2019_94_RIGHT

nCoV-2019_2

TTTGGCAATGTTGTTCCTTGAGG

23

43.48

60.18

nCoV-2019_95_LEFT

nCoV-2019_1

TGAGGGAGCCTTGAATACACCA

22

50

61.1

nCoV-2019_95_RIGHT

nCoV-2019_1

CAGTACGTTTTTGCCGAGGCTT

22

50

61.95

nCoV-2019_96_LEFT

nCoV-2019_2

GCCAACAACAACAAGGCCAAAC

22

50

61.82

nCoV-2019_96_RIGHT

nCoV-2019_2

TAGGCTCTGTTGGTGGGAATGT

22

50

61.36

nCoV-2019_97_LEFT

nCoV-2019_1

TGGATGACAAAGATCCAAATTTCAAAGA

28

32.14

60.22

nCoV-2019_97_RIGHT

nCoV-2019_1

ACACACTGATTAAAGATTGCTATGTGAG

28

35.71

60.17

nCoV-2019_98_LEFT

nCoV-2019_2

AACAATTGCAACAATCCATGAGCA

24

37.5

60.5

nCoV-2019_98_RIGHT

nCoV-2019_2

TTCTCCTAAGAAGCTATTAAAATCACATGG

30

33.33

60.01