23/31 were CD4+; five were CD8+; one (C-24) was a mixture; Two clusters (C-30 and C-31) were CD4−CD8−
C-20:contained a subset of CD4−CD8− and CD8+ cells expressing innate-like T cell markers, including ZBTB16 and CD161 and CD26 surface proteins
one central memory cluster (C-25) and distinct GZMK+ (C-28) and GZMB+ (C-29) effector subsets, reflecting different cytotoxicities
high expression of HLA-DR and CD38 surface protein and proliferation-associated MKI67 (C-15 and C-27) represent chronically activated cells
TH17 cells (C-12, CCR6+ and RORC)
type 1 helper T cells (TH1; C-17, CXCR3+ and IFNG and TBX21)
heterogeneous continuum of intermediate TH1/TH17 states (C-13, C-16 and C-19) with varying degrees of CXCR3, CCR6, CCR5 and CD161 surface protein expression and RORC and TBX21 expression
CD161+ subset of type 2 helper T (TH2) cells (C-14), described as pathogenic, with higher expression of allergy-associated HPGDS and IL17RB
a subset of FOXP3+ Treg cells (C-5) expressing higher CCR6 surface protein and CTLA4 and RORC than other Treg cells
记忆T细胞的状态因人口特征和环境而异
发现:年龄因素的影响最大;性别因素与T细胞状态也高度相关;type 2 helper T (TH2) 细胞状态在冬天(季节因素)搜集的样本中最为丰富