分享是一种态度
此教程演示分析存储在Seurat对象中的RNA速率定量。参数基于RNA速率教程[1]。如果您在工作中使用Seurat,请引用:
提前安装好如下3个R包。
加载所需R包
library(Seurat)
library(velocyto.R)
library(SeuratWrappers)
# If you don't have velocyto's example mouse bone marrow dataset, download with the CURL command
# curl::curl_download(url = 'http://pklab.med.harvard.edu/velocyto/mouseBM/SCG71.loom', destfile
# = '~/Downloads/SCG71.loom')
ldat <- ReadVelocity(file = "~/Downloads/SCG71.loom")
bm <- as.Seurat(x = ldat)
bm <- SCTransform(object = bm, assay = "spliced")
bm <- RunPCA(object = bm, verbose = FALSE)
bm <- FindNeighbors(object = bm, dims = 1:20)
bm <- FindClusters(object = bm)
bm <- RunUMAP(object = bm, dims = 1:20)
bm <- RunVelocity(object = bm, deltaT = 1, kCells = 25, fit.quantile = 0.02)
ident.colors <- (scales::hue_pal())(n = length(x = levels(x = bm)))
names(x = ident.colors) <- levels(x = bm)
cell.colors <- ident.colors[Idents(object = bm)]
names(x = cell.colors) <- colnames(x = bm)
show.velocity.on.embedding.cor(emb = Embeddings(object = bm, reduction = "umap"), vel = Tool(object = bm,
slot = "RunVelocity"), n = 200, scale = "sqrt", cell.colors = ac(x = cell.colors, alpha = 0.5),
cex = 0.8, arrow.scale = 3, show.grid.flow = TRUE, min.grid.cell.mass = 0.5, grid.n = 40, arrow.lwd = 1,
do.par = FALSE, cell.border.alpha = 0.1)
[1]
RNA速率教程: http://pklab.med.harvard.edu/velocyto/notebooks/R/SCG71.nb.html