**全基因组DNA甲基化测序(Whole Genome Bisulfite Sequencing,WGBS)**是 DNA 甲基化研究的金标准,它通过 Bisulfite 处理和全基因组 DNA 测序结合的方式,对整个基因组上的甲基化情况进行分析,具有单碱基分辨率,可精确评估单个 C 碱基的甲基化水平,构建全基因组精细甲基化图谱。数据量非常大。
有文章比较这3个技术:Empirical comparison of reduced representation bisulfite sequencing and Infinium BeadChip reproducibility and coverage of DNA methylation in humans,感兴趣的都是可以自由阅读,提高自己哈。甲基化信号的主要分析也是 差异甲基化区域(DMRs)与 DMR 相关差异基因。
于 2020 Aug 发表在cancer research 杂志的文章,标题是:《STAT3 and GR Cooperate to Drive Gene Expression and Growth of Basal-Like Triple-Negative Breast Cancer》, DOI: 10.1158/0008-5472.CAN-20-1379
如下所示:
To identify regulatory regions specific to basal-like breast cancer, reduced representation bisulfite sequencing (RRBS) was performed on 28 breast cancer cell lines (18 basal-like and 10 luminal) in order to measure DNA methylation across the genome.
Of the 479,746 CG positions in the genome with at least 10x coverage in each cell line, 3,748 CGs were significantly differentially methylated (Linear Regression Benjamini-Hochberg adjusted p< 0.05) between basal-like and luminal cell lines