但是最近看到了一个文章:《Single-cell Transcriptomic Architecture Unraveling the Complexity of Tumor Heterogeneity in Distal Cholangiocarcinoma》样品队列是 4 patients with dCCA,取肿瘤部位和癌旁正常组织,分别独立做10x的单细胞转录组数据 :
4 treatment-naïve dCCA samples
3 matched adjacent normal biliary duct tissues
第一层次分群也是肿瘤上皮细胞,基质细胞(内皮,成纤维等),免疫细胞(淋巴系的T, NK 和B,髓系的巨噬和单核),如下所示:
epithelial cells (3946 cells; 12.78%; marked with EPCAM),;
endothelial cells (3760 cells; 12.18%; marked with VWF),;
fibroblast cells (1481 cells; 4.80%; marked with COL1A1),;
tissue stem cells (671 cells; 2.17%; marked with NOTCH3)
macrophages (2610 cells; 8.46%; marked with CD68),;
neutrophils (2508 cells; 8.13%; with marker gene FCGR3B),;
natural killer (NK) cells (1789 cells; 5.80%; marked with CD7),;
T cells (10,592 cells; 34.32%; with marker gene CD2),;
B cells (1382 cells; 4.48%; marked with CD79A),;
nerve cells (1251 cells; 4.05%; with marker gene NGFR),;
mast cells (870 cells; 2.82%; with marker gene TPSB2),;