问图像路径是有效的，但pyautogui.locateOnScreen无法读取图像文件？

EN

import cv2
import pyautogui

if __name__ == '__main__':
    template = cv2.imread("Images/新增.png", 0)
    pos = pyautogui.locateOnScreen("Images/新增.png")

[ WARN:0@1.236] global D:\a\opencv-python\opencv-python\opencv\modules\imgcodecs\src\loadsave.cpp (239) cv::findDecoder imread_('Images/新增.png'): can't open/read file: check file path/integrity
[ WARN:0@1.296] global D:\a\opencv-python\opencv-python\opencv\modules\imgcodecs\src\loadsave.cpp (239) cv::findDecoder imread_('Images/新增.png'): can't open/read file: check file path/integrity
Traceback (most recent call last):
  File "E:\Python代码\HealthCheckRunner\HealthCheck.py", line 82, in <module>
    pos = pyautogui.locateOnScreen("Images/新增.png")
  File "F:\HealthCheckRunner\lib\site-packages\pyautogui\__init__.py", line 175, in wrapper
    return wrappedFunction(*args, **kwargs)
  File "F:\HealthCheckRunner\lib\site-packages\pyautogui\__init__.py", line 213, in locateOnScreen
    return pyscreeze.locateOnScreen(*args, **kwargs)
  File "F:\HealthCheckRunner\lib\site-packages\pyscreeze\__init__.py", line 373, in locateOnScreen
    retVal = locate(image, screenshotIm, **kwargs)
  File "F:\HealthCheckRunner\lib\site-packages\pyscreeze\__init__.py", line 353, in locate
    points = tuple(locateAll(needleImage, haystackImage, **kwargs))
  File "F:\HealthCheckRunner\lib\site-packages\pyscreeze\__init__.py", line 207, in _locateAll_opencv
    needleImage = _load_cv2(needleImage, grayscale)
  File "F:\HealthCheckRunner\lib\site-packages\pyscreeze\__init__.py", line 170, in _load_cv2
    raise IOError("Failed to read %s because file is missing, "
OSError: Failed to read Images/新增.png because file is missing, has improper permissions, or is an unsupported or invalid format

Process finished with exit code 1

pos = pyautogui.locateOnScreen("E:/Python代码/HealthCheckRunner/Images/新增.png")
--------------------------
OSError: Failed to read E:/Python代码/HealthCheckRunner/Images/新增.png because file is missing, has improper permissions, or is an unsupported or invalid format