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社区首页 >问答首页 >通过RSEM分析获得的XXX.cnt信息

通过RSEM分析获得的XXX.cnt信息
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Stack Overflow用户
提问于 2020-09-02 08:36:46
回答 1查看 67关注 0票数 0

经过RSEM-1.3.3分析后,我在新创建的"XXX.stat“目录中获得了一个"XXX.cnt”。

下面显示的是XXX.cnt的内容。

代码语言:javascript
复制
0 2726098 0 2726098
1534055 1192043 1993977
9793897 1
0       0
1       732121
2       410181
3       513309
4       610475
5       90206
6       81551
7       63620
8       44947
9       33029
10      21745
11      22282
12      21545
13      13324
14      17247
.
.
.

这些数字意味着什么?

首先要感谢您的好意。

EN

回答 1

Stack Overflow用户

回答已采纳

发布于 2020-09-04 14:53:34

RSEM目录下的"cnt_file_description.txt“中描述了每个字段的格式和含义。

http://deweylab.github.io/RSEM/rsem-calculate-expression.html#OUTPUT

https://github.com/bli25broad/RSEM_tutorial

这是文字记录。

代码语言:javascript
复制
# '#' marks the start of comments (till the end of the line) 
# *.cnt file contains alignment statistics based purely on the alignment results obtained from aligners

N0 N1 N2 N_tot   
#  N0, number of unalignable reads; N1, number of alignable reads; N2, number of filtered reads due to too many alignments; N_tot = N0 + N1 + N2     
    
nUnique nMulti nUncertain   
# nUnique, number of reads aligned uniquely to a gene; nMulti, number of reads aligned to multiple genes; nUnique + nMulti = N1;                             
# nUncertain, number of reads aligned to multiple locations in the given reference sequences, which include isoform-level multi-mapping reads

nHits read_type             
# nHits, number of total alignments.                         
# read_type: 0, single-end read, no quality score; 1, single-end read, with quality score; 2, paired-end read, no quality score; 3, paired-end read, with quality score
        
# The next section counts reads by the number of alignments they have. Each line contains two values separated by a TAB character. The first value is number of alignments. 'Inf' refers to reads filtered due to too many alignments. The second value is the number of reads that contain such many alignments
    
0                          N0
...
number_of_alignments       number_of_reads_with_that_many_alignments
...                           
Inf                        N2
票数 0
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页面原文内容由Stack Overflow提供。腾讯云小微IT领域专用引擎提供翻译支持
原文链接:

https://stackoverflow.com/questions/63697139

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