<asp:GridView ID="GridView1" runat="server" AutoGenerateColumns="False" OnRowDataBound="GridView1_RowDataBound">
    <Columns>
        <asp:TemplateField>
            <ItemTemplate>
                <asp:CheckBox ID="CheckBox1" runat="server" Checked='<%# Eval("CheckboxColumn") %>' />
            </ItemTemplate>
        </asp:TemplateField>
        <asp:BoundField DataField="Column1" HeaderText="Column 1" />
        <asp:BoundField DataField="Column2" HeaderText="Column 2" />
        <!-- 其他列 -->
    </Columns>
</asp:GridView>

protected void GridView1_RowDataBound(object sender, GridViewRowEventArgs e)
{
    if (e.Row.RowType == DataControlRowType.DataRow)
    {
        CheckBox checkBox = (CheckBox)e.Row.FindControl("CheckBox1");
        // 获取复选框列的值
        bool isChecked = (bool)DataBinder.Eval(e.Row.DataItem, "CheckboxColumn");
        checkBox.Checked = isChecked;
    }
}