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    centrifuge软件以及数据库

    基于鸟枪法(Shotgun Sequencing)的高通量测序已经走过 10 多年,在宏基因组领域的应用也超过 10 年,在这 10 多年里,基于二代测序高通量的特性,在宏基因组,16S 测序中已经取得了非常大的进展。然而,二代测序读长短、建库周期长、无法实时测序等技术特点,依然限制了宏基因组数据分析的发展。尤其是读长短,只有不到 2X300bp,比对唯一性差,会造成一对多的比对,并且短读长无法得到好的拼接效果,无法直接从宏基因组中拼接出完整细菌基因组等。而这些技术缺点,通过新一代的纳米孔测序可以很好的解决,纳米孔诸多的优点为宏基因组研究带来了新的突破,下面我们来总结一下 nanopore 测序技术在宏基因组中的应用。

    02

    grep、sed、awk练习题

    文件:datafile Steve Blenheim:238-923-7366:95 Latham Lane, Easton, PA 83755:11/12/56:20300 Betty Boop:245-836-8357:635 Cutesy Lane, Hollywood, CA 91464:6/23/23:14500 Igor Chevsky:385-375-8395:3567 Populus Place, Caldwell, NJ 23875:6/18/68:23400 Norma Corder:397-857-2735:74 Pine Street, Dearborn, MI 23874:3/28/45:245700 Jennifer Cowan:548-834-2348:583 Laurel Ave., Kingsville, TX 83745:10/1/35:58900 Jon DeLoach:408-253-3122:123 Park St., San Jose, CA 04086:7/25/53:85100 Karen Evich:284-758-2857:23 Edgecliff Place, Lincoln, NB 92086:7/25/53:85100 Karen Evich:284-758-2867:23 Edgecliff Place, Lincoln, NB 92743:11/3/35:58200 Karen Evich:284-758-2867:23 Edgecliff Place, Lincoln, NB 92743:11/3/35:58200 Fred Fardbarkle:674-843-1385:20 Parak Lane, DeLuth, MN 23850:4/12/23:780900 Fred Fardbarkle:674-843-1385:20 Parak Lane, DeLuth, MN 23850:4/12/23:780900 Lori Gortz:327-832-5728:3465 Mirlo Street, Peabody, MA 34756:10/2/65:35200 Paco Gutierrez:835-365-1284:454 Easy Street, Decatur, IL 75732:2/28/53:123500 Ephram Hardy:293-259-5395:235 CarltonLane, Joliet, IL 73858:8/12/20:56700 James Ikeda:834-938-8376:23445 Aster Ave., Allentown, NJ 83745:12/1/38:45000 Barbara Kertz:385-573-8326:832 Ponce Drive, Gary, IN 83756:12/1/46:268500 Lesley Kirstin:408-456-1234:4 Harvard Square, Boston, MA 02133:4/22/62:52600 William Kopf:846-836-2837:6937 Ware Road, Milton, PA 93756:9/21/46:43500 Sir Lancelot:837-835-8257:474 Camelot Boulevard, Bath, WY 28356:5/13/69:24500 Jesse Neal:408-233-8971:45 Rose Terrace, San Francisco, CA 92303:2/3/36:25000 Zippy Pinhead:834-823-8319:2356 Bizarro Ave., Farmount, IL 84357:1/1/67:89500 Arthur Putie:923-835-8745:23 Wimp Lane, Kensington, DL 38758:8/31/69:126000 Popeye Sailor:156-454-3322:945 Bluto Street, Anywhere, USA 29358:3/19/35:22350 Jose Santiago:385-898-8357:38 Fife Way, Abilene, TX 39673:1/5/58:95600 Tommy Savage:408-724-0140:1222 Oxbow Court, Sunnyvale, CA 94087:5/19/66:34200 Yukio Takeshida:387-827-1095:13 Uno Lane, Ashville, NC 23556:7/1/29:57000 Vinh Tranh:438-910-7449:8235 Maple Street, Wilmington,

    02

    Python 学习入门(10)—— 时间

    Python格式化日期时间的函数为datetime.datetime.strftime();由字符串转为日期型的函数为:datetime.datetime.strptime(),两个函数都涉及日期时间的格式化字符串,列举如下: %a     Abbreviated weekday name %A     Full weekday name %b     Abbreviated month name %B     Full month name %c     Date and time representation appropriate for locale %d     Day of month as decimal number (01 - 31) %H     Hour in 24-hour format (00 - 23) %I     Hour in 12-hour format (01 - 12) %j     Day of year as decimal number (001 - 366) %m     Month as decimal number (01 - 12) %M     Minute as decimal number (00 - 59) %p     Current locale's A.M./P.M. indicator for 12-hour clock %S     Second as decimal number (00 - 59) %U     Week of year as decimal number, with Sunday as first day of week (00 - 51) %w     Weekday as decimal number (0 - 6; Sunday is 0) %W     Week of year as decimal number, with Monday as first day of week (00 - 51) %x     Date representation for current locale %X     Time representation for current locale %y     Year without century, as decimal number (00 - 99) %Y     Year with century, as decimal number %z, %Z     Time-zone name or abbreviation; no characters if time zone is unknown %%     Percent sign

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